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KMID : 0525720170220030156
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Journal of Chitin and Chitosan
2017 Volume.22 No. 3 p.156 ~ p.161
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Characterization of Cellulase from Bacillus sp. TH1 Isolated from Environmental Soil
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Kang Dong-Wook
Park Chang-Su
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Abstract
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To screen cellulolytic microorganism, we carried out screening from environmental soil, which is sampled at Kyeongsans-si, Kyeonbuk, Korea. One strain, which is named as Bacillus sp. TH1, was isolated as a cellulolytic microorganism due to its ability of enzyme activity for carboxymethylcellulose (CM-cellulose). In the analysis of 16S rRNA gene sequence from the strain, the isolated strain showed 99% of identity with Bacillus sp. R-11590 (GenBank AJ438301) therefore we named the strain Bacillus sp. TH1. The cellulase from Bacillus sp. TH1 showed the highest activities at pH 5.0 and 50°C conditions. In the investigation of pH and temperature stability, the cellulase from Bacillus sp. TH1 stabled pH 4.0~5.5 range and until 45°C for 30 min perfectly. And the cellulase from Bacillus sp. TH1 for the various cellulosic substrates such as CM-cellulose, alkali swollen cellulose, Avicel, Alpha-cellulose, and Sigmacell-cellulose has the most high activity for CM-cellulose. In the degradation of swollen cellulose, the most proper enzyme and substrate concentrations were 3.0 U/ml of enzyme and 1.0% of CMcellulose, respectively.
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KEYWORD
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Environmental soil, Screening, Bacillus sp., Cellulase, Characterization
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